|Specifics: This is for 35th Anniversary comic|
Lady Slimer was Slimer's partner-in-crime as it were, and joined him on his joyride. Slimer drove past the Ghostbusters in front of The Mercado building in the Times Square area. The second time she was seen, she seems to have gotten closer to Slimer. They ended up both going through the portal and the Divide to the World of the Dead as part of the Ghostbusters' plan to blow up the reactors atop Ecto-1's roof rack in order to reverse the polarity of the portal and force all the ghosts back through. Slimer and Lady Slimer were propelled into the air atop the Ecto-1's seat cushions. They exchanged looks and laughed then fell back down into the portal.
During the ghost retrieval mission in the prime dimension, Lady Slimer sat next to Slimer 80-C as he drove a shuttle from the Warehouse to the Firehouse to drop off the Ghostbusters of Dimension 11-W, Ghostbusters of Dimension 68-M, and Ghostbusters of Dimension 68-Q.
While Slimer was an androgynous blob creature with no defining gender characteristics, Lady Slimer is evidently female, with red lipstick and blonde hair with a pink bow tie.
- Lady Slimer is a homage to Mandy Pepperidge (played by Mary Louise Weller), from film "Animal House".
- Slimer is loosely based on actor John Belushi, which as Bluto marries the Mandy character.
- Lady Slimer was based on the wife of Looney Tunes character the Tasmanian Devil.
- On June 21, 2016, Robin Shelby confirmed she had a role in the movie. 
- On July 9, 2016, it was reported from the live cast of the movie premiere that Robin Shelby provided the voice of Lady Slimer.
- The shot of Slimer and Lady Slimer shooting up into the air out of the Portal is a nod to a scene in "Die Hard 2" when John McClane ejects out a jetliner just before several grenades detonate.
- Lady Slimer makes a non-canon cameo on Credits Page of Ghostbusters 101 #1.
- On the Subscription Cover of Ghostbusters 101 #5, on the right bottom is Lady Slimer.
- On Cover RI of Ghostbusters Crossing Over Issue #4, Lady Slimer appears.
- ↑ EMPIRE Online "Empire Podcast: Ghostbusters Spoiler Special with Paul Feig" 11:51-12:00 7/27/16 Paul Feig says: "She's kind of based on uh remember when the Tasmanian Devil had met his wife, 'Yeah, make it look like Slimer with a wig on and lipstick'."
- ↑ Robin Shelby Facebook (Must be logged in) 6/21/16
- ↑ Ghostbusters News "Robin Shelby’s role in new Ghostbusters revealed" 7/9/16
- ↑ Paul Feig (2016). Ghostbusters (2016 Movie) Extended Edition - Writer & Director Commentary (2016) (Blu-Ray ts. 01:55:03-01:55:05). Sony Pictures. Paul Feig says: "This is a tribute to Die Hard 2."