The Unicorns are mythological creatures who are antagonists of Sir Bruce.
After he was freed from his painting, Sir Bruce directed his Bully Boys to awaken everything in the Cloisters. A few paintings, however, held his enemies - the Unicorns. The Unicorns were rounded up and barricaded into a room. While on the run from other Cloisters Manifestations, Ray Stantz and Winston Zeddemore found the room by accident. Winston figured out they were allies and the two rode the Unicorns out. Sir Bruce and his Bully Boys gave chase. Outside the Cloisters, one of the Unicorns challenged Bruce to a duel and won. After Bruce was trapped, the Unicorns returned to their paintings.
- On page 17 of Ghostbusters Volume 2 Issue #14, the Unicorn Tapestry makes a non-canon cameo on the wall of the hotel room above Winston Zeddemore and Tiyah Zeddemore.
- On the regular cover of Ghostbusters International #5, to the right of the Mona Lisa is a Unicorn Tapestry seen towards the end of "Hard Knight's Day" when the paintings return to their proper places in the Cloisters.
- On page five of Ghostbusters International #7, a Unicorn Tapestry makes a non-canon cameo in panel 5 behind Kylie Griffin.
- On page 12 of Ghostbusters 101 #1, Zoe Zawadzki mentions Unicorns.